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Effect of pEGFP-survivin on GBC-SD cell growth and chemotherapy sensitivity

Hanbin SHEN MD, Bin ZHANG MBA, Song ZHAO BM, Qichang ZHENG MD, Jianping GONG MD, Xiaotang CAI BM,

Frontiers of Medicine 2009, Volume 3, Issue 4, Pages 485-490 doi: 10.1007/s11684-009-0089-3

Abstract: investigate the effect of survivin shRNA on chemotherapy resistance in human gallbladder carcinoma GBC-SDThe viability of human gallbladder carcinoma GBC-SD, GBC-SD/enhanced green fluorescent protein (EGFP), and GBC-SD/survivin cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideCell viability was decreased significantly in GBC-SD/survivin cells, and survivin expression was decreasedactivity in all the cells was increased at first and then decreased, but the caspase-3 activity in GBC-SD

Keywords： survivin shRNA chemotherapy sensibility GBC-SD